Detection of Parasite and Virus in Grow Out of Hybrid Groupers (Epinephelus fuscoguttatus vs Epinephelus lanceolatus)
Abstract
The declining productivity of cantang grouper is influenced by the presence of pathogenic infections from viruses and parasites. Parasites can attack cantang grouper at all stages, while Iridovirus and Viral Nervous Necrosis (VNN) infections are serious diseases that can cause mass death in cantang grouper aquaculture. The purpose of this study was to obtain information on the incidence of disease in grouper rearing in ponds and in concrete tanks infected with parasitic and viral pathogens. Detection of parasites was carried out by taking 5 samples of cantang grouper kept in concrete tanks and in ponds with a density of 3 fish/m2 using macroscopic and microscopic methods, while iridovirus and VNN observations used the PCR method. The results showed that parasitic attacks were found during rearing, namely Epistylis sp. with a prevalence of 100% and an intensity of 146.6, Trichodina sp. with 100% prevalence and intensity. 104.4, Caligus sp., Diplectanum sp. During the study, no Iridovirus and VNN were detected in cantang groupers.
References
Adibrata, S., Yusuf, M., & Manullang, C. (2020). Ectoparasite prevalences of grouper fish (Epinephelus fuscogutatus x Epinephelus polyphekadion) cultured in floating net cages. Ilmu Kelautan: Indonesian Journal of Marine Sciences, 25(1), 23–30. https://doi.org/10.14710/ik.ijms.25.1.23-30
Amira Masitha, Uun Yanuharab, A. M. S. H. (2019). In-Vivo Test of Chlorella vulgaris Extract for Induction into Heat Shock Proteins of Cantang Grouper (Ephinephelus fuscoguttatus-lanceolatus) Infected by Viral Nervous Necrosis. JFMR-Journal of Fisheries and Marine Research, 3(1), 22–31. https://doi.org/10.21776/ub.jfmr.2019.003.01.3
Arya, K., Sudewa, M., & Suratmi, S. (2018). Teknik Pemeliharaan Parasit (Lintah, Zeylanicobdella arugamensis) Dalam Sistem Air Mengalir Dan Tergenang Ketut. Buletin Teknik Litkayasa Akuakultur, 16(1), 65–68.
Assefa, A., & Abunna, F. (2018). Maintenance of Fish Health in Aquaculture: Review of Epidemiological Approaches for Prevention and Control of Infectious Disease of Fish. Veterinary Medicine International, 2018. https://doi.org/10.1155/2018/5432497
Dedi, D. (2018). Pengaruh Pemberian Hormon Tiroksin Pada Pakan Pellet Megami Terhadap Pertumbuhan Benih Ikan Kerapu Cantang (Epinephelus fuscoguttatus-Lanceolatus). Intek Akuakultur. https://doi.org/10.31629/intek.v2i2.536
Galingging, Y. T., Sudaryatama, P. E., Julyantoro, P. G. S., Waskita, A. H., Lestari, A. T., & Nurlita, W. (2022). Identification of Viral Nervous Necrosis (VNN) in Grouper Fish (Epinephelus sp.) by Reverse Transcriptase-Polymerase Chain Reaction. Advances in Tropical Biodiversity and Environmental Sciences, 6(1), 1. https://doi.org/10.24843/atbes.2022.v06.i01.p01
Grabda. (1991). Marine fish parasitology: An outline. Marine Fish Parasitology - an Outline, 7(12), 357. https://doi.org/10.1016/0169-4758(91)90222-a
Hart, P. J., & Reynolds, J. D. (Eds.). (2008). Handbook of Fish Biology and Fisheries, Volume 2: Fisheries. John Wiley & Sons.
Kurita, J., Nakajima, K., Hirono, I., & Aoki, T. (1998). Polymerase chain reaction (PCR) amplification of DNA of red sea bream iridovirus (RSIV). Fish Pathology, 33(1), 17–23. https://doi.org/10.3147/jsfp.33.17
Kurniawan, & Murwantoko. (2015). Prevalensi, Intensitas Dan Identifikasi Molekuler Ektoparasit Pada Kerapu Di Karamba Jaring Apung Teluk Pegametan Kabupaten Buleleng.
Koesharyani, 1., K. Mahardika, K. Sugama, and K. yuasa. 2001. lridovirus penyebab kematian pada budidaya ikan kerapu lumpur, Epinephelus coioides. Deteksi menggunakan polymerase chain reaction (PCR). Prosiding Seminar Teknologi Budidaya Laut dan Pengembangan Sea Farming di lndonesia. Dep.Kelautan dan Perikanan bekerja sama dengan JICA, p.228--234.
Mahardika, K., Mastuti, I., Syahidah, D., Ismi, S., & Zafran. (2021). Disease surveillance of cultured marine fish in the North of Bali, Indonesia. IOP Conference Series: Earth and Environmental Science, 890(1), 0–14. https://doi.org/10.1088/1755-1315/890/1/012035
Mahasri, G., Hafidloh, U., Pratama, F. P., Rahmawan, D., Subekti, S., Wulansari, P. D., & Amin, M. (2020). Prevalence, intensity and histopathology of Zeylanicobdella arugamensis infestation on groupers reared on different aquaculture systems. Journal of Fish Diseases, 43(10), 1133–1143. https://doi.org/10.1111/jfd.13219
Murwantoko, M., Negoro, S. L. C., Isnansetyo, A., & Zafran, Z. (2018). Short Communication: Identification of marine leech and assessment of its prevalence and intensity on cultured hybrid groupers (Epinephelus sp.). Biodiversitas Journal of Biological Diversity. https://doi.org/10.13057/biodiv/d190529
OIE. (2009). Manual diagnostic test for aquatic animals. chapter 2.3.7 red sea bream iridoviral disease. Fish Pathology, 52(2), 57–62. https://doi.org/10.3147/jsfp.52.57
Purwantoro, E. D., & Prasetio, E. (2021). Identifikasi Dan Prevalensi Ektoparasit Pada Ikan Napoleon (Cheilinus undulatus) Yang Dibudidayakan Di Keramba Jaring Tancap (Kjt) Perairan Sedanau Kabupaten Natuna. Technical Sciences and Technologies, 3(2(24)), 235–243. https://doi.org/10.25140/2411-5363-2021-2(24)-235-243
Puspitasari D. 2017. Teknik pembesaran ikan kerapu cantik (Epinephelus sp.) pada Keramba jaring apung di Balai Perikanan Budidaya Air Payau (BPBAP) Situbondo, Jawa Timur. Universitas Airlangga.
Rimmer, M. A., Whittington, R. J., Becker, J. A., Dhand, N. K., Hick, P. M., Mahardika, K., Sembiring, S., Ismi, S., Zafran, Z., Haryanti, Roza, D., Kusumawati, D., Asih, Y. N., Sudewi, Mastuti, I., Wardana, I. K., Murwantoko, Fusianto, C. K., & Fachry, M. E. (2019). Improving fish health management and production protocols in marine finfish aquaculture in Indonesia and Australia. Project number: ACIAR/FIS/2010/101.
Rochmad, A. N. (2020). Teknik Pembesaran Ikan Kerapu Hibrida Cantang (Epinephelus fuscoguttatus × Epinephelus lanceolatus) pada Karamba Jaring Apung. Jurnal Biosains Pascasarjana, 22(1), 29. https://doi.org/10.20473/jbp.v22i1.2020.29-36
Rijal SS, GDAP Bayuaji. 2021. Penentuan kesesuaian lokasi marikultur ikan kerapu di Sumatera Utara, Indonesia menggunakan Google Earth Engine. Journal of Fisheries and Marine Research, 5(2): 357-367
St-Hilaire, S., Cheng, T. H., Chan, S. C. H., Leung, C. F., Chan, K. M., Lim, K. Z., Furtado, W., & Bastos Gomes, G. (2021). Emamectin Benzoate Treatment of Hybrid Grouper Infected With Sea Lice in Hong Kong. Frontiers in Veterinary Science, 8(February), 1–9. https://doi.org/10.3389/fvets.2021.646652
Smith, S. & Schwarz, M. (2019). Dealing with Trichodina and Trichodina-like species Commercial Fish & Shellfish Technology Fact Sheet What are Trichodina and. Virginia Tech, 205.
Suhermanto, A., Gardenia, L., Hermawan, T., Taqwa, F. H., & Poltak, H. (2022). Sosialisasi Program Dari Papua Untuk Semua Sebagai Upaya Meningkatkan Pengetahuan Masyarakat Tentang Pentingnya Vaksin Dan Vaksinasi." JMM (Jurnal Masyarakat Mandiri) 6, no. 4 (2022): 2927-2939.
Susanti, E. (2016). Identeksi Virus TSV (Taura Syndrome Virus) Pada Udang Vannamei (Litopenaeus vannamei) Di Kabupaten Mempawah Hilir Dengan Metode PCR (Polymerase Chain Reaction). (Skripsi, Fakultas Perikanan dan Ilmu Kelautan, Universitas Muhammadiyah Pontianak).
Yu, Q., Liu, M., Wei, S., Xiao, H., Wu, S., Qin, X., Shi, D., Li, S., Wang, T., & Li, P. (2019). Isolation of nervous necrosis virus from hybrid grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus) cultured in Guangxi, China. Fish Pathology, 54(1), 16–19. https://doi.org/10.3147/jsfp.54.16
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